Exosc3^tm1.1Uba
Targeted Allele Detail

Summary

• Symbol: Exosc3^tm1.1Uba
• Name: exosome component 3; targeted mutation 1.1, Uttiya Basu
• MGI ID: MGI:5702728
• Synonyms: Exosc3^COIN
• Gene: Exosc3 Location: Chr4:45316613-45320616 bp, - strand Genetic Position: Chr4, 23.84 cM, cytoband B2
• Alliance: Exosc3^tm1.1Uba page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:217084
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac or C57BL/6NTac

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change, Reporter)
• Mutation: Insertion
• Mutation details: Lox2372 and loxP sites were inserted in intron 1 and an antisense oriented conditional inversion (COIN) module, a second set of lox2372 and loxP sites, and an FRT flanked neo cassette were inserted in intron 3 The two lox sites are in reverse complementation orientation, thus cre-mediated recombination will irreversibly invert the intervening sequence. The COIN module consists of a splice acceptor site and a T2A-GFP-pA cassette. Flp-mediated recombination removed the drug selection cassette. (J:217084)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Exosc3 Mutation: 13 strains or lines available

Notes

"The...targeting vector was electroporated into ROSA26^CreERt2/+, 129S6/SvEvTac x C57BL/6Tac hybrid ES cells." (J:217084)

References

• Original: J:217084 Pefanis E, et al., Noncoding RNA transcription targets AID to divergently transcribed loci in B cells. Nature. 2014 Oct 16;514(7522):389-93
• All: 3 reference(s)