Pik3c2a^tm1Ehi
Targeted Allele Detail

Summary

• Symbol: Pik3c2a^tm1Ehi
• Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha; targeted mutation 1, Emilio Hirsch
• MGI ID: MGI:5700367
• Gene: Pik3c2a Location: Chr7:115936500-116042684 bp, - strand Genetic Position: Chr7, 61.62 cM
• Alliance: Pik3c2a^tm1Ehi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:226733
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A lacZ gene and loxP-flanked neomycin resistance cassette was inserted in-frame into the endogenous locus. RT-PCR analysis on RNA derived from homozygous embryos showed that a transcript from this allele is undetectable. A complete loss of encoded protein was shown with antibodies to both the N and C terminus of the protein. (J:226733)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pik3c2a Mutation: 98 strains or lines available

References

• Original: J:226733 Franco I, et al., PI3K class II alpha controls spatially restricted endosomal PtdIns3P and Rab11 activation to promote primary cilium function. Dev Cell. 2014 Mar 31;28(6):647-58
• All: 3 reference(s)