Sdk1^{tm1.1(cre/ERT2)Jrs}
Targeted Allele Detail

Summary

• Symbol: Sdk1^{tm1.1(cre/ERT2)Jrs}
• Name: sidekick cell adhesion molecule 1; targeted mutation 1.1, Joshua R Sanes
• MGI ID: MGI:5698881
• Synonyms: Sdk1^ce
• Gene: Sdk1 Location: Chr5:141227245-142201341 bp, + strand Genetic Position: Chr5, 79.81 cM
• Alliance: Sdk1^{tm1.1(cre/ERT2)Jrs} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:225578
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Null/knockout, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Sdk1^{tm1.1(cre/ERT2)Jrs} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Sdk1 (MGI:2444413)

• Mutation details: The first coding exon was replaced with an inducible cre recombinase gene (cre/ERT2) epitope tagged with three tandem copies of haemagglutinin (HA). Cre-mediated recombination removed the floxed neomycin resistance cassette. Immunohistochemistry confirmed the absence of protein expression in the retina from P30. (J:225578)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Sdk1 (mouse) Summary of all recombinase alleles driven by Sdk1. Alliance mouse Sdk1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sdk1 Mutation: 111 strains or lines available

References

• Original: J:225578 Krishnaswamy A, et al., Sidekick 2 directs formation of a retinal circuit that detects differential motion. Nature. 2015 Aug 27;524(7566):466-70
• All: 3 reference(s)