Cr2^tm3.1Jhws
Targeted Allele Detail

Summary

• Symbol: Cr2^tm3.1Jhws
• Name: complement receptor 2; targeted mutation 3.1, John Weiss
• MGI ID: MGI:5698828
• Synonyms: Cr2KO
• Gene: Cr2 Location: Chr1:194819119-194859024 bp, - strand Genetic Position: Chr1, 98.44 cM
• Alliance: Cr2^tm3.1Jhws page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:223195
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutations: Insertion, Intragenic deletion
• Mutation details: This mutant allele was engineered to remove the alternative splice site required for the expression of the Cr2 isoform but leave the Cr1 isoform intact. A construct containing a cDNA fusion of the SCRs 18 (thus removing the alternative splice junction) was ligated to the testes specific self-deleting pACN neomycin resistance gene and flanked by Cr2 homologous DNA. This construct replaced exons 1-8 of the endogenous gene. The neomycin selection cassette was removed in the germline. This allele expresses Cr1 on B cells but is deficient for the full length Cr2 protein. An alternative splice within the Cr2 gene created a truncated product that encoded a novel protein termed iCr2 that was expressed on the surface of these cells. (J:223195)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cr2 Mutation: 84 strains or lines available

References

• Original: J:223195 Donius LR, et al., Generation of a novel Cr2 gene allele by homologous recombination that abrogates production of Cr2 but is sufficient for expression of Cr1. Immunobiology. 2014 Jan;219(1):53-63
• All: 1 reference(s)