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Transgene Detail
Symbol: Tg(Actb-GFP,-BBS4)B4T78Vcs
Name: transgene insertion B4T78, Val C Sheffield
MGI ID: MGI:5697974
Synonyms: BBS4tg
Transgene: Tg(Actb-GFP,-BBS4)B4T78Vcs  Location: unknown  
Strain of Origin:  C57BL/6J x SJL/J
Transgene Type:    Transgenic (Humanized sequence, Inserted expressed sequence)
Mutation:    Insertion
Tg(Actb-GFP,-BBS4)B4T78Vcs expresses 1 gene
Mutation detailsHuman BBS4 and a localization and purification (LAP) tag (GFP followed by a cleavage site for Tobacco Etch Virus (TEV) protease and an S-tag) were placed under the control of the beta actin (Actb) promoter in a transgenic vector. Expression of the transgene is highest in testis, followed by brain, adipose, eye, heart and very slight expression is seen in trachea, lungs and bone. No expression is observed in the liver or skin. Immunofluorescence assays confirm that localization of transgene expression is similar to endogenous BBS4, such that it localizes to the centrosome and cilia. (J:199518)
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Original:  J:199518 Chamling X, et al., Ectopic expression of human BBS4 can rescue Bardet-Biedl syndrome phenotypes in Bbs4 null mice. PLoS One. 2013;8(3):e59101
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.17
The Jackson Laboratory