Col1a1^{tm2.1(tetO-IDH2*R172K)Nbar}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm2.1(tetO-IDH2*R172K)Nbar}
• Name: collagen, type I, alpha 1; targeted mutation 2.1, Nabeel Bardeesy
• MGI ID: MGI:5695654
• Synonyms: Tet-R172K
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm2.1(tetO-IDH2*R172K)Nbar} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:217109
• Parent Cell Line: C2 (Jaenisch) (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Humanized sequence, Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutations: Insertion, Nucleotide substitutions
• Col1a1^{tm2.1(tetO-IDH2*R172K)Nbar} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  IDH2 (3418)

  Idh2	(MGI:96414)

• Mutation details: A cassette containing a CMV/tetO promoter and a human IDH2 cDNA with an R172K mutation was inserted in the 3' end of the gene. An FRT clanked selection cassette was also inserted and then removed by flp mediated recombination. (J:217109)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 166 strains or lines available

Notes

C2 (Jaenisch) ES cells are derived from ES cell line v6.5.

References

• Original: J:217109 Saha SK, et al., Mutant IDH inhibits HNF-4alpha to block hepatocyte differentiation and promote biliary cancer. Nature. 2014 Sep 4;513(7516):110-4
• All: 1 reference(s)