Col1a1^{tm1.1(tetO-IDH2*R140Q)Nbar}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1.1(tetO-IDH2*R140Q)Nbar}
• Name: collagen, type I, alpha 1; targeted mutation 1.1, Nabeel Bardeesy
• MGI ID: MGI:5695652
• Synonyms: Tet-R140Q
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1.1(tetO-IDH2*R140Q)Nbar} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:217109
• Parent Cell Line: C2 (Jaenisch) (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Humanized sequence, Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutations: Insertion, Nucleotide substitutions
• Col1a1^{tm1.1(tetO-IDH2*R140Q)Nbar} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  IDH2 (3418)

  Idh2	(MGI:96414)

• Col1a1^{tm1.1(tetO-IDH2*R140Q)Nbar} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	tetO

• Mutation details: A cassette containing a CMV/tetO promoter and a human IDH2 cDNA with a p.R140Q mutation was inserted into the FRT site flanked docking site downstream of the gene. An FRT site flanked selection cassette was also inserted and then removed by Flp-mediated recombination. (J:217109)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 166 strains or lines available

Notes

C2 (Jaenisch) ES cells are derived from ES cell line v6.5.

References

• Original: J:217109 Saha SK, et al., Mutant IDH inhibits HNF-4alpha to block hepatocyte differentiation and promote biliary cancer. Nature. 2014 Sep 4;513(7516):110-4
• All: 1 reference(s)