Kcnmb2^tm1.2Clin
Targeted Allele Detail

Summary

• Symbol: Kcnmb2^tm1.2Clin
• Name: potassium large conductance calcium-activated channel, subfamily M, beta member 2; targeted mutation 1.2, Christopher J Lingle
• MGI ID: MGI:5694812
• Synonyms: beta2 KO
• Gene: Kcnmb2 Location: Chr3:31956656-32254329 bp, + strand Genetic Position: Chr3, 15.48 cM
• Alliance: Kcnmb2^tm1.2Clin page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:225860
• Parent Cell Line: B6/BLU (ES Cell)
• Strain of Origin: C57BL/6NTac-Tg(HBB-lacZ)ALey/Ley

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A targeting vector was designed to insert a loxP site upstream of exon 1 and a second loxP site downstream of exon 1 followed by an FRT-flanked neomycin resistance (neo) cassette and a third loxP site downstream of exon 1. Cre-mediated recombination removed exon 1 and the neo cassette. Immunoprecipitation of cerebellum, adrenal medulla and cerebral cortex tissues confirmed that no protein was expressed in mutant mice. (J:225860)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kcnmb2 Mutation: 17 strains or lines available

References

• Original: J:225860 Martinez-Espinosa PL, et al., Knockout of the BK beta2 subunit abolishes inactivation of BK currents in mouse adrenal chromaffin cells and results in slow-wave burst activity. J Gen Physiol. 2014 Oct;144(4):275-95
• All: 2 reference(s)