Tg(CAG-GCaMP2)2Mik
Transgene Detail
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Symbol: |
Tg(CAG-GCaMP2)2Mik |
Name: |
transgene insertion 2, Michael I Kotlikoff |
MGI ID: |
MGI:5693370 |
Synonyms: |
pCAGGS-GCaMP2 |
Transgene: |
Tg(CAG-GCaMP2)2Mik Location: unknown
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Alliance: |
Tg(CAG-GCaMP2)2Mik page
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Transgene Type: |
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Transgenic (Reporter) |
Mutation: |
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Insertion
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Mutation details: This 3.7 kbp transgene has a loxP site, the CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), the GCaMP2 sequence (described below), a polyadenylation signal and a second loxP site. Founder line 2 was identified with high sensor expression.
The genetically encoded calcium indicator GCaMP2 is a calcium-sensing molecule composed of a 35 aa polyHis plasmid leader sequence (RSET; essential for thermal stability), a 13 residue peptide of chicken smooth muscle myosin light chain kinase (M13), a circularly permutated EGFP (cpEGFP; aa 149-238 followed by aa 1-144 [with mutations listed below]), and a rat calmodulin DNA fragment (CaM; aa 2-148). The cpEGFP mutations result in increased brightness (D180Y and V93I), thermal stability (V163A and S175G) and GFP dimerization prevention (A206K).
(J:101977)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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Original: |
J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017; |
All: |
1 reference(s) |
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