Tg(Gt(ROSA)26Sor-Gak*)1Legr
Transgene Detail

Summary

• Symbol: Tg(Gt(ROSA)26Sor-Gak*)1Legr
• Name: transgene insertion 1, Lois Greene
• MGI ID: MGI:5693250
• Synonyms: C62, GAK-C62
• Transgene: Tg(Gt(ROSA)26Sor-Gak*)1Legr Location: unknown
• Alliance: Tg(Gt(ROSA)26Sor-Gak*)1Legr page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Gt(ROSA)26Sor-Gak*)1Legr expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Gak (MGI:2442153)

• Mutation details: The transgene, constructed in the pBROAD3 vector (InvivoGen), encodes the 560 C-terminal amino acids of the mouse GAK (cyclin G associated kinase) protein, which comprise its clathrin binding domain and its HSPA8- (also called HSC70-) interacting J domain. Expression of the partial Gak cDNA is driven by a modified Gt(ROSA)26Sor promoter whose 5' untranslated region (5' UTR) is interrupted by an engineered intron; the expressed sequence is followed by the human beta-globin 3' UTR and polyadenylation signal. Immunoblot analysis using polyclonal rabbit antibodies to GAK demonstrated expression of the truncated protein in all tissues examined: brain, heart, kidney, liver and lung. (J:225348)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:225348 Park BC, et al., The clathrin-binding and J-domains of GAK support the uncoating and chaperoning of clathrin by Hsc70 in the brain. J Cell Sci. 2015 Oct 15;128(20):3811-21
• All: 1 reference(s)