Tg(VDR*L233S,luc)T807Pike
Transgene Detail

Summary

• Symbol: Tg(VDR*L233S,luc)T807Pike
• Name: transgene insertion T807, J Wesley Pike
• MGI ID: MGI:5662065
• Synonyms: hVDR^L233S
• Transgene: Tg(VDR*L233S,luc)T807Pike Location: unknown
• Alliance: Tg(VDR*L233S,luc)T807Pike page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(VDR*L233S,luc)T807Pike expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  VDR (7421)

  Vdr	(MGI:103076)

• Mutation details: The BAC clone RP11-89H19 containing the entire human VDR gene and its surrounding intergenic segments (82 kb of the 5'-intergenic region and 48 kb of the 3'-intergenic region) was engineered by BAC recombineering techniques to contain a hemagglutinin tag (HA) at the translation start site of the human VDR gene and a cassette containing an IRES-driven luciferase reporter and a TK promoter-driven neomycin resistance gene selection mechanism in the 3' untranslated region of the human VDR gene. The leucine to serine (L233S) mutation was introduced through nucleotide mutagenesis (CTG to TCG) at codon 233. This mutation compromises the 1,25(OH)2D3-binding capacity of the VDR. Three lines were generated; T805, T806, and T807. T807 is the high expressing line. (J:218709)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:218709 Lee SM, et al., A humanized mouse model of hereditary 1,25-dihydroxyvitamin D-resistant rickets without alopecia. Endocrinology. 2014 Nov;155(11):4137-48
• All: 2 reference(s)