Bcat1^tm1.2Eaa
Targeted Allele Detail

Summary

• Symbol: Bcat1^tm1.2Eaa
• Name: branched chain aminotransferase 1, cytosolic; targeted mutation 1.2, Elitsa A Ananieva
• MGI ID: MGI:5660825
• Gene: Bcat1 Location: Chr6:144939561-145021883 bp, - strand Genetic Position: Chr6, 77.27 cM
• Alliance: Bcat1^tm1.2Eaa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:216027
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 0.5 kb genomic region including exon 6 was flanked with loxP sites and a floxed neo cassette was inserted via homologous recombination. Cre mediated recombination removed the neo cassette and exon 6. Western blot analysis confirmed the absence of protein expression in brains of homozygous mice. (J:216027)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bcat1 Mutation: 164 strains or lines available

References

• Original: J:216027 Ananieva EA, et al., Cytosolic branched chain aminotransferase (BCATc) regulates mTORC1 signaling and glycolytic metabolism in CD4+ T cells. J Biol Chem. 2014 Jul 4;289(27):18793-804
• All: 2 reference(s)