Gt(ROSA)26Sor^{tm1(CAG-GCaMP3)Dbe}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-GCaMP3)Dbe}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Dwight E Bergles
• MGI ID: MGI:5659933
• Synonyms: R26-lsl-GCaMP3
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-GCaMP3)Dbe} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:215494
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The Rosa-CAG-LSL-GCaMP3-WPRE targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked STOP cassette, a GCaMP3 cDNA, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a BGH polyA signal, and an FRT-flanked Neo cassette. The GCaMP3 fusion gene contains a cDNA sequence encoding the chicken smooth muscle M13 fragment of myosin light chain kinase, a circularly permutated EGFP (amino acid residues 149-238 followed by amino acid residues 1-144 (including M153K, V163A, S175G, D180Y, T203V, A206K, and V93I mutations to increase dynamic range and baseline fluorescence), and a DNA fragment encoding amino acid residues 2-148 of the rat calmodulin (modified to harbor the N60D mutation to increase the fluorescence change for small calcium transients). (J:215494)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 942 strains or lines available

References

• Original: J:215494 Paukert M, et al., Norepinephrine controls astroglial responsiveness to local circuit activity. Neuron. 2014 Jun 18;82(6):1263-70
• All: 18 reference(s)