Mdm2^tm2.1Ypz
Targeted Allele Detail

Summary

• Symbol: Mdm2^tm2.1Ypz
• Name: transformed mouse 3T3 cell double minute 2; targeted mutation 2.1, Yanping Zhang
• MGI ID: MGI:5649075
• Synonyms: Mdm2^Y487A
• Gene: Mdm2 Location: Chr10:117524780-117546663 bp, - strand Genetic Position: Chr10, 66.32 cM, cytoband C1-C3
• Alliance: Mdm2^tm2.1Ypz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:214218
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A loxP site flanked neomycin resistance gene cassette was inserted into intron 11 and a tyrosine codon 487 (TAC) in exon 12 was changed to alanine (GCA) (p.Y487A). Cre-mediated recombination removed the neo cassette. This mutation disrupts the E3 ligase activity without altering binding to MDM4. (J:214218)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mdm2 Mutation: 54 strains or lines available

References

• Original: J:214218 Tollini LA, et al., Regulation of p53 by Mdm2 E3 ligase function is dispensable in embryogenesis and development, but essential in response to DNA damage. Cancer Cell. 2014 Aug 11;26(2):235-47
• All: 1 reference(s)