Lama5^tm2.1Jhm
Targeted Allele Detail

Summary

• Symbol: Lama5^tm2.1Jhm
• Name: laminin, alpha 5; targeted mutation 2.1, Jeffrey H Miner
• MGI ID: MGI:5648730
• Gene: Lama5 Location: Chr2:179818166-179867652 bp, - strand Genetic Position: Chr2, 102.7 cM
• Alliance: Lama5^tm2.1Jhm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:189883
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to flank exons 15-21, which encodes 40% of domain V, consisting of laminin EGF-like repeats. Upon removal of the sequence, splicing from exon 14 to exon 22 should result in an out of frame transcript. The neo cassette used for selection purposes was removed by flp-mediated recombination. Cre-mediated recombination removed exons 15 through 21. (J:189883)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lama5 Mutation: 153 strains or lines available

References

• Original: J:189883 Kim ST, et al., Functional consequences of cell type-restricted expression of laminin alpha5 in mouse placental labyrinth and kidney glomerular capillaries. PLoS One. 2012;7(7):e41348
• All: 2 reference(s)