Tg(Igf2/LacZ,H19)YZ20Aco
Transgene Detail

Summary

• Symbol: Tg(Igf2/LacZ,H19)YZ20Aco
• Name: transgene insertion YZ20, Justin Ainscough
• MGI ID: MGI:5648554
• Transgene: Tg(Igf2/LacZ,H19)YZ20Aco Location: unknown
• Alliance: Tg(Igf2/LacZ,H19)YZ20Aco page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F2

Transgene description

• Transgene Type: Transgenic (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(Igf2/LacZ,H19)YZ20Aco expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	H19 (MGI:95891)
  mouse	Igf2 (MGI:96434)

• Mutation details: The 130 kb YAC transgene, YZ, contains the Igf2 and H19 genomic regions and in which the Igf2 coding sequence was interrupted with the lacZ reporter gene. The YZ YAC extends from promoter 1 of Igf2 to 35 kb downstream of H19, and includes the differentially methylated region around exons 5 to 6 of Igf2, the unmethylated intergenic hypersensitive region, A6-A4, the differentially methylated regions around H19 and the endoderm-specific enhancers downstream of H19. In addition, the 1kb conserved intergenic region is flanked with LoxP sites. LacZ activity shows spatial expression of Igf2 in a subset of tissues. The imprinted H19 gene is only expressed when transmitted through the maternal germline. After paternal transmission the gene is repressed. Line YZ20 carries 3 copies of the transgene. (J:43322)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

References

• Original: J:64089 Ainscough JF, et al., A skeletal muscle-specific mouse igf2 repressor lies 40 kb downstream of the gene. Development. 2000 Sep;127(18):3923-30
• All: 2 reference(s)