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F3tm1Fct
Targeted Allele Detail
Nomenclature
Symbol: F3tm1Fct
Name: coagulation factor III; targeted mutation 1, Felix C Tanner
MGI ID: MGI:5647876
Synonyms: asTFKI
Gene: F3  Location: Chr3:121723537-121735048 bp, + strand  Genetic Position: Chr3, 52.94 cM
Yolk sac abnormalities in F3tm1Fct/F3tm1Fct embryos

Show the 1 phenotype image(s) involving this allele.

Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:216519
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Modified isoform(s))
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsThe open reading frame was replaced with one encoding the soluble alternatively spliced form and a loxP flanked neomycin resistance cassette. After homologous recombination into ES cells, the floxed neomycin resistance cassette was removed by transfection with a Cre expression plasmid. mRNA expression of the soluble form was 5.9-fold higher in homozygous mutant embryos relative to wild-type embryos, whereas mRNA expression of the full-length form was undetectable in homozygous mutant embryos. Western blot analysis revealed comparable protein expression of the soluble form in E9.5 homozygous mutant and wild-type embryos. (J:216519)
Generation of the F3tm1Fct allele
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any F3 Mutation:  1 strain or line available
References
Original:  J:216519 Sluka SH, et al., Alternatively spliced tissue factor is not sufficient for embryonic development. PLoS One. 2014;9(5):e97793
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
03/24/2020
MGI 6.15
The Jackson Laboratory