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Targeted Allele Detail
Symbol: F3tm1Fct
Name: coagulation factor III; targeted mutation 1, Felix C Tanner
MGI ID: MGI:5647876
Synonyms: asTFKI
Gene: F3  Location: Chr3:121723537-121735048 bp, + strand  Genetic Position: Chr3, 52.94 cM
Yolk sac abnormalities in F3tm1Fct/F3tm1Fct embryos

Show the 1 phenotype image(s) involving this allele.

Germline Transmission:  Earliest citation of germline transmission: J:216519
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Modified isoform(s))
Mutations:    Insertion, Intragenic deletion
Mutation detailsThe open reading frame was replaced with one encoding the soluble alternatively spliced form and a loxP flanked neomycin resistance cassette. After homologous recombination into ES cells, the floxed neomycin resistance cassette was removed by transfection with a Cre expression plasmid. mRNA expression of the soluble form was 5.9-fold higher in homozygous mutant embryos relative to wild-type embryos, whereas mRNA expression of the full-length form was undetectable in homozygous mutant embryos. Western blot analysis revealed comparable protein expression of the soluble form in E9.5 homozygous mutant and wild-type embryos. (J:216519)
Generation of the F3tm1Fct allele
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any F3 Mutation:  1 strain or line available
Original:  J:216519 Sluka SH, et al., Alternatively spliced tissue factor is not sufficient for embryonic development. PLoS One. 2014;9(5):e97793
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.15
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