Tg(CAMK2A-KCNIP3*,-lacZ)1Cnbc
Transgene Detail

Summary

• Symbol: Tg(CAMK2A-KCNIP3*,-lacZ)1Cnbc
• Name: transgene insertion 1, Centro Nacional de Biotecnologia
• MGI ID: MGI:5646261
• Synonyms: EFmDREAM tg
• Transgene: Tg(CAMK2A-KCNIP3*,-lacZ)1Cnbc Location: unknown
• Alliance: Tg(CAMK2A-KCNIP3*,-lacZ)1Cnbc page

Transgene origin

• Strain of Origin: (C57BL/6 CBA)F1

Transgene description

• Transgene Type: Transgenic (Dominant negative, Inserted expressed sequence)
• Mutation: Insertion
• Tg(CAMK2A-KCNIP3*,-lacZ)1Cnbc expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  KCNIP3 (30818)

  Kcnip3

  (MGI:1929258)

• Mutation details: The cDNA encoding human KCNIP3 (DREAM) with two amino-acid substitutions each at EFhands 2, 3 and 4 (EFmDREAM) was inserted downstream of the human CaMKII promoter. The cDNA was followed by an IRES and the LacZ reporter gene. The amino acid substitutions rendered the mutant insensitive to Ca2+, and thus can block the Ca2+dependent derepression function of DREAM and other KChIP proteins endogenously expressed. (J:103733)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

References

• Original: J:103733 Savignac M, et al., Transcriptional repressor DREAM regulates T-lymphocyte proliferation and cytokine gene expression. EMBO J. 2005 Oct 19;24(20):3555-64
• All: 7 reference(s)