Gt(ROSA)26Sor^{tm2(SNAPCaaX)Phep}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(SNAPCaaX)Phep}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Paul A Heppenstall
• MGI ID: MGI:5645795
• Synonyms: SnapCaaX Ai
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(SNAPCaaX)Phep} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:222844
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: This is a Cre-dependent allele that conditionally expresses a SNAP-tag protein containing a membrane associated CaaX consensus sequence. The SNAP-tag construct consists of an engineered O6-alkylguanine-DNA alkyltransferase that can be specifically and irreversibly labeled with O6-benzylguanine (BG) derivatives. Cre-mediated removal of the stop cassette induces expression of the SNAP-CaaX protein. The Cre-dependent conditional expression of SNAPCaax inner membrane protein allows chemical labelling of proteins in specific cell types in vivo. (J:222844)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 944 strains or lines available

Notes

Mice were healthy, displayed normal fertility and did not exhibit any obvious defects.

References

• Original: J:222844 Yang G, et al., Genetic targeting of chemical indicators in vivo. Nat Methods. 2015 Feb;12(2):137-9
• All: 2 reference(s)