Tg(ADCYAP1R1)1Sshe
Transgene Detail
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Symbol: |
Tg(ADCYAP1R1)1Sshe |
Name: |
transgene insertion 1, Sanbing Shen |
MGI ID: |
MGI:5645747 |
Synonyms: |
A149 |
Transgene: |
Tg(ADCYAP1R1)1Sshe Location: unknown
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Alliance: |
Tg(ADCYAP1R1)1Sshe page
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Strain of Origin: |
C57BL/6 or CBA/Ca
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Transgene Type: |
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Transgenic (Inserted expressed sequence) |
Mutation: |
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Insertion
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Tg(ADCYAP1R1)1Sshe expresses
1 gene
Transgene expresses:
Organism |
Expressed Gene |
Homolog in Mouse |
Note |
human |
ADCYAP1R1 (117) |
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Mutation details: The transgenic construct comprised the 130-kb RPCI PAC clone 204D22, containing the human adenylate cyclase activating polypeptide 1 receptor 1 (ADCYAP1R1; also called PAC1) gene, modified by insertion of an internal ribosomal entry site (IRES) followed by the lacZ coding sequence (IRES-lacZ-p[A] cassette) into the 3' untranslated region (3' UTR) downstream of the human gene's polyadenylation signal. Semi-quantitative PCR of genomic DNA from heterozygous mice determined that transgenic line 1 (also called A149) carries six copies of the transgene. RT-PCR of RNA from embryonic day 10.5 (E10.5) embryos revealed a high level of transgene expression.
(J:111714)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 2 strains available
Cell Lines: 0 lines available
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"PAC1-overexpressing mice were generated in a mixed genetic background of CBA/Ca: C57Bl/6...." ( J:179496)
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Original: |
J:111714 Lang B, et al., Expression of the human PAC1 receptor leads to dose-dependent hydrocephalus-related abnormalities in mice. J Clin Invest. 2006 Jul;116(7):1924-34 |
All: |
2 reference(s) |
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