Tg(tetO/Prnp-GCaMP3)600Cai
Transgene Detail

Summary

• Symbol: Tg(tetO/Prnp-GCaMP3)600Cai
• Name: transgene insertion 600, Huaibin Cai
• MGI ID: MGI:5644521
• Synonyms: GC, tetO-GCaMP3
• Transgene: Tg(tetO/Prnp-GCaMP3)600Cai Location: unknown
• Alliance: Tg(tetO/Prnp-GCaMP3)600Cai page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Inducible, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: The transgenic construct contains the Tet-responsive element and mouse prion protein non-coding exons 1-2 upstream of a GCaMP3 cDNA sequence, followed by mouse prion protein non-coding exon 3 and polyadenylation signal. The GCaMP3 fusion gene cDNA sequence contains a cDNA sequence encoding the chicken smooth muscle M13 fragment of myosin light chain kinase, a circularly permutated EGFP (amino acid residues 149-238] followed by amino acid residues 1-144 (including M153K, V163A, S175G, D180Y, T203V, A206K and V93I mutations to increase dynamic range and baseline fluorescence), and a DNA fragment encoding amino acid residues 2-148 of the rat calmodulin (modified to harbor the N60D mutation to increase the fluorescence change for small calcium transients). (J:222395)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:222395 Sgobio C, et al., Optogenetic measurement of presynaptic calcium transients using conditional genetically encoded calcium indicator expression in dopaminergic neurons. PLoS One. 2014;9(10):e111749
• All: 2 reference(s)