Sema3d^{tm1a(KOMP)Bzsh}
Targeted Allele Detail

Summary

• Symbol: Sema3d^{tm1a(KOMP)Bzsh}
• Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D; targeted mutation 1a, Bin Zhou
• MGI ID: MGI:5638222
• Synonyms: Sema3d^Lacz
• Gene: Sema3d Location: Chr5:12433352-12638915 bp, + strand Genetic Position: Chr5, 3.97 cM, cytoband A2
• Alliance: Sema3d^{tm1a(KOMP)Bzsh} page
• IMPC: Sema3d gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:220957
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 12555324 of Chromosome 5 upstream of the critical exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 5 at position 12556103. The critical exon 5 is thus flanked by loxP sites. (J:220957)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sema3d Mutation: 53 strains or lines available

References

• Original: J:220957 Tian X, et al., Subepicardial endothelial cells invade the embryonic ventricle wall to form coronary arteries. Cell Res. 2013 Sep;23(9):1075-90
• All: 1 reference(s)