Cacna1itm1Alu
Targeted Allele Detail
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| Symbol: |
Cacna1itm1Alu |
| Name: |
calcium channel, voltage-dependent, alpha 1I subunit; targeted mutation 1, Anita Luthi |
| MGI ID: |
MGI:5637591 |
| Synonyms: |
Cav3.3- |
| Gene: |
Cacna1i Location: Chr15:80171439-80282480 bp, + strand Genetic Position: Chr15, 37.85 cM, cytoband E2
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| Alliance: |
Cacna1itm1Alu page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:175607
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| Parent Cell Line: |
E14.1 (ES Cell)
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| Strain of Origin: |
129P2/OlaHsd
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| Allele Type: |
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Targeted (Null/knockout) |
| Mutations: |
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Insertion, Intragenic deletion
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Cacna1itm1Alu involves 1 genes/genome features (Cacna1i)
View all
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Mutation details: A 6.8-kb genomic DNA segment containing exons 11-21 has been replaced with a cassette containing a EN2-SA-internal ribosomal entry site (IRES) followed by a beta-galactosidase-neomycin phosphotransferase (-neo) fusion gene (betageo). A loxP-flanked selection cassette containing Pgk-neo and MC1 tk genes was deleted by cre recombinase. The deleted segment encodes the predicted IIS2 and IIS4 transmembrane domains and the third predicted intracellular domain; the modification also is predicted to cause a frame-shift upon splicing of exon 10 to exon 22. RT-PCR analysis of RNA from cerebellum, cortex, olfactory bulb, and thalamus of homozygous mutant mice confirmed that no full-length Cacna1i mRNA is generated.
(J:175607)
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| Inheritance: |
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Recessive |
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Cacna1i Mutation: |
76 strains or lines available
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| Original: |
J:175607 Astori S, et al., The CaV3.3 calcium channel is the major sleep spindle pacemaker in thalamus. Proc Natl Acad Sci U S A. 2011 Aug 16;108(33):13823-8 |
| All: |
4 reference(s) |
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Analysis Tools
