Clstn1^tm1.1Uge
Targeted Allele Detail

Summary

• Symbol: Clstn1^tm1.1Uge
• Name: calsyntenin 1; targeted mutation 1.1, Urs Gerber
• MGI ID: MGI:5637427
• Synonyms: Cst-1 KO
• Gene: Clstn1 Location: Chr4:149670925-149733356 bp, + strand Genetic Position: Chr4, 79.91 cM, cytoband E1
• Alliance: Clstn1^tm1.1Uge page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:212386
• Parent Cell Line: ART B6-3, B6-3 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 17 and a floxed neo cassette was inserted downstream of exon 19. Cre mediated recombination removed exons 17-19 and the neo cassette. Immunoblot analysis of whole-brain homogenates using an antibody raised against the cytoplasmic segment revealed absence of the functionally active full-length transport form as well as absence of the C-terminal cytosolic segment in homozygous mutant mice. An antibody raised against the N-terminal cadherin-like domains detected a truncated form in homozygous and heterozygous mutant mice that is slightly shorter than the ectodomain in wild-type mice, the predominant form that normally accumulates after juxtamembrane cleavage in the extracellular compartment. Because of the absence of the transmembrane and cytosolic segment, this fragment lacks the kinesin-docking function of full-length protein. (J:212386)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Clstn1 Mutation: 58 strains or lines available

References

• Original: J:212386 Ster J, et al., Calsyntenin-1 regulates targeting of dendritic NMDA receptors and dendritic spine maturation in CA1 hippocampal pyramidal cells during postnatal development. J Neurosci. 2014 Jun 25;34(26):8716-27
• All: 1 reference(s)