Fzd3^{tm1a(EUCOMM)Agof}
Targeted Allele Detail

Summary

• Symbol: Fzd3^{tm1a(EUCOMM)Agof}
• Name: frizzled class receptor 3; targeted mutation 1a, Andre M Goffinet
• MGI ID: MGI:5637347
• Gene: Fzd3 Location: Chr14:65429898-65499912 bp, - strand Genetic Position: Chr14, 34.09 cM
• Alliance: Fzd3^{tm1a(EUCOMM)Agof} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:212261
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph, Reporter)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 65476881 of Chromosome 14 upstream of exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 65477799. Exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. (J:212261, J:215832)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fzd3 Mutation: 32 strains or lines available

References

• Original: J:212261 Qu Y, et al., Genetic evidence that Celsr3 and Celsr2, together with Fzd3, regulate forebrain wiring in a Vangl-independent manner. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):E2996-3004
• All: 4 reference(s)