Shox2^{tm1.1(Shox2*)Jfm}
Targeted Allele Detail

Summary

• Symbol: Shox2^{tm1.1(Shox2*)Jfm}
• Name: SHOX homeobox 2; targeted mutation 1.1, James F Martin
• MGI ID: MGI:5636788
• Synonyms: Shox2^HA
• Gene: Shox2 Location: Chr3:66879060-66889104 bp, - strand Genetic Position: Chr3, 30.76 cM, cytoband E3-F1
• Alliance: Shox2^{tm1.1(Shox2*)Jfm} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:212095
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Shox2^{tm1.1(Shox2*)Jfm} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Shox2 (MGI:1201673)

• Mutation details: A Flag-HA-Shox2a-DsRed-polyA cassette and an FRT flanked neo cassette were inserted at the start site in exon 1 via homologous recombination. Flp mediated recombination removed the neo cassette. The Shox2a cDNA encodes an N-terminal FLAG-HA- tagged full length Shox2 gene. The Shox2a cDNA has a heterologous 3'UTR releasing the gene from endogenous miRNA control. Immunofluorescence analysis indicates aberrant elevated expression of the mutant protein at E16.5 in the atrial septum. (J:212095)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Shox2 Mutation: 20 strains or lines available

References

• Original: J:212095 Wang J, et al., Pitx2-microRNA pathway that delimits sinoatrial node development and inhibits predisposition to atrial fibrillation. Proc Natl Acad Sci U S A. 2014 Jun 24;111(25):9181-6
• All: 7 reference(s)