Tg(Ckm-Prkaa2*D157A)1Ljg
Transgene Detail

Summary

• Symbol: Tg(Ckm-Prkaa2*D157A)1Ljg
• Name: transgene insertion 1, Laurie J Goodyear
• MGI ID: MGI:5636710
• Synonyms: alpha2i TG, AMPKalpha2i TG
• Transgene: Tg(Ckm-Prkaa2*D157A)1Ljg Location: unknown
• Alliance: Tg(Ckm-Prkaa2*D157A)1Ljg page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Ckm-Prkaa2*D157A)1Ljg expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  rat

  Prkaa2 (78975)

  Prkaa2

  (MGI:1336173)

  Transgene codes for a protein with an aspartic acid to alanine mutation.

• Mutation details: The transgenic construct contains a rat cDNA containing the Prkaa2 gene modified by site-directed mutagenesis to contain the D157A mutation under the control of the muscle creatine kinase promoter. The mutation changes the aspartic acid at residue 157 to alanine thus inactivating the catalytic alpha 2 subunit. The inactive alpha 2 subunit is tagged with a hemaglutinin epitope at the amino terminus. Expression of endogenous Prkaa2 is detectable only after very long exposure times and transgene expression occurs in skeletal muscle. (J:104111)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:104111 Fujii N, et al., AMP-activated protein kinase alpha2 activity is not essential for contraction- and hyperosmolarity-induced glucose transport in skeletal muscle. J Biol Chem. 2005 Nov 25;280(47):39033-41
• All: 11 reference(s)