Col1a1^{tm1.1(CAG-Sirt2)Jmi}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1.1(CAG-Sirt2)Jmi}
• Name: collagen, type I, alpha 1; targeted mutation 1.1, Jeffrey Milbrandt
• MGI ID: MGI:5636689
• Synonyms: SIRT2tg
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1.1(CAG-Sirt2)Jmi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:211655
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Col1a1^{tm1.1(CAG-Sirt2)Jmi} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Sirt2 (MGI:1927664)

• Mutation details: The SIRT2-Tg flox-STOP-flox construct was designed to insert (from 5' to 3') an FRT site, a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG; from the pCAGGS vector), a loxP-flanked transcriptional STOP cassette, the mouse sirtuin 2 (Sirt2) cDNA sequence, and a PGK-hygro-polyA sequence (with a second FRT site between the PGK and hygro) into the 3' UTR of the mouse collagen A1 (Col1a1) locus. Cre mediated recombination removed the floxed STOP construct. (J:177468)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 166 strains or lines available

References

• Original: J:211655 North BJ, et al., SIRT2 induces the checkpoint kinase BubR1 to increase lifespan. EMBO J. 2014 Jul 1;33(13):1438-53
• All: 4 reference(s)