Lgr5^{tm1.1(cre/ERT2)Npa}
Targeted Allele Detail

Summary

• Symbol: Lgr5^{tm1.1(cre/ERT2)Npa}
• Name: leucine rich repeat containing G protein coupled receptor 5; targeted mutation 1.1, Novartis Pharma AG
• MGI ID: MGI:5635821
• Synonyms: Lgr5-EGFP-IRES-CreERT2, Lgr5ki
• Gene: Lgr5 Location: Chr10:115286219-115423685 bp, - strand Genetic Position: Chr10, 63.51 cM
• Alliance: Lgr5^{tm1.1(cre/ERT2)Npa} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:212415
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Inducible, Null/knockout, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Lgr5^{tm1.1(cre/ERT2)Npa} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Lgr5 (MGI:1341817)

• Mutation details: The targeting construct replaced the first 22 amino acids with an EGFP reporter gene, IRES, inducible cre recombinase fusion (cre/ERT2), polyadenylation sequence and FRT-flanked neomycin resistance cassette. Flp-mediated recombination removed the selection cassette. In situ hybridization confirmed the absence of transcript expression in the intestin, skin and kidney. (J:212415)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Lgr5 (mouse) Summary of all recombinase alleles driven by Lgr5. Comparative matrix view of recombinase activities Alliance mouse Lgr5 gene page

Expression

In Mice Carrying this Mutation:

122 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lgr5 Mutation: 57 strains or lines available

References

• Original: J:212415 Kinzel B, et al., Functional roles of Lgr4 and Lgr5 in embryonic gut, kidney and skin development in mice. Dev Biol. 2014 Jun 15;390(2):181-90
• All: 5 reference(s)