Cia51^DBA/1J
QTL Variant Detail

Summary

• QTL variant: Cia51^DBA/1J
• Name: collagen induced arthritis QTL 51; DBA/1J
• MGI ID: MGI:5635558
• QTL: Cia51 Location: Chr14:66103893-68785736 bp Genetic Position: Chr14, Syntenic

Variant origin

• Strain of Specimen: DBA/1J

Variant description

• Allele Type: QTL

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:201946

Collagen-induced arthritis (CIA) is the most commonly used model of rheumatoid arthritis (RA) in mice. In this study a known locus, Cia6, and a genomic region of Chromosome 14 were dissected using a partial advanced intercross (PAI) and a collection of congenic strains.

The congenics used for the PAI [[B10.D1-(D6Mit86-D6Mit188) H2^q/Rhd, B10.D1-(D6Mit268-D6Mit65) H2^q/Rhd and B10.D1-(D14Mit212-D14Mit160) H2^q/Rhd] were produced as speed congenics by marker assisted backcrossing of the susceptible DBA/1 (DQ) mice to the resistant, recipient strain C57BL/10.Q for seven generations. Four hundred and eighty four male, mice were tested in arthritis experiments. Seven markers were either linked to arthritis in single locus scans or involved in significant interactions detected in a two-locus scan.

To confirm and further define the indicated locus regions subcongenic strains were produced by backcrossing selected DBA/1 fragments from the PAI to the B10.Q background. Two hundred and fifty six heterozygous, male mice and 273 heterozygous, female mice were tested for CIA susceptibility and antibody production. The peak markers and marker intervals found in the congenic mice correlated well with the loci found in the PAI. Three new loci were detected on Chromosome 6 and four new loci on Chromosome 14.

In the PAI, Cia51 was detected on chromosome 14 in the single locus scan at peak marker D14Mit235 where it affected incidence of disease (LOD 3.0), onset (LOD 4.7) and severity (LOD 2.6). Cia51 interacted with Cia46 to increase the severity of arthritis and with Cia50 to increase the production of IgG in the PAI.

In the congenic mice Cia51, peak marker at D14Mit39, increased the production of IgG1 (p=0.008) and IgG3 (p=0.02) in the female mice. Antibody titreswere also slightly higher in the male mice and both sexes tended towards higher disease scores. Figure 1 displays this QTL mapping between D14Mit102 and D14Mit39.

References

• Original: J:201946 Popovic M, et al., Identification of new loci controlling collagen-induced arthritis in mouse using a partial advanced intercross and congenic strains. Scand J Immunol. 2008 Oct;68(4):405-13
• All: 1 reference(s)