Fen1^tm2.1Bhsh
Targeted Allele Detail

Summary

• Symbol: Fen1^tm2.1Bhsh
• Name: flap structure specific endonuclease 1; targeted mutation 2.1, Binghui Shen
• MGI ID: MGI:5629667
• Synonyms: Fen1^E359K
• Gene: Fen1 Location: Chr19:10176496-10181533 bp, - strand Genetic Position: Chr19, 6.54 cM
• Alliance: Fen1^tm2.1Bhsh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:218627
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S1/Sv

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Single point mutation
• Mutation details: The coding sequence was replaced with a cDNA with a G to A point mutation that results in an amino acid substitution of lysine for glutamic acid at position 359 (E359K). Cre-mediated recombination removed a floxed neomycin resistance cassette inserted upstream of the modified cDNA. Western blot analysis revealed a similar expression of FEN1 in wild-type and homozygous mutant mice. (J:218627)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fen1 Mutation: 30 strains or lines available

References

• Original: J:218627 Chung L, et al., The FEN1 E359K germline mutation disrupts the FEN1-WRN interaction and FEN1 GEN activity, causing aneuploidy-associated cancers. Oncogene. 2015 Feb 12;34(7):902-11
• All: 1 reference(s)