Dars2^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Dars2^{tm1b(KOMP)Wtsi}
• Name: aspartyl-tRNA synthetase 2 (mitochondrial); targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5629584
• Gene: Dars2 Location: Chr1:160868171-160898228 bp, - strand Genetic Position: Chr1, 69.75 cM
• Alliance: Dars2^{tm1b(KOMP)Wtsi} page
• IMPC: Dars2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:210635
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 160892554 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 160893282. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). (J:210635)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dars2 Mutation: 37 strains or lines available

References

• Original: J:210635 Dogan SA, et al., Tissue-specific loss of DARS2 activates stress responses independently of respiratory chain deficiency in the heart. Cell Metab. 2014 Mar 4;19(3):458-69
• All: 1 reference(s)