Pirt^tm2Xzd
Targeted Allele Detail

Summary

• Symbol: Pirt^tm2Xzd
• Name: phosphoinositide-interacting regulator of transient receptor potential channels; targeted mutation 2, Xinzhong Dong
• MGI ID: MGI:5620948
• Synonyms: Pirt-GCaMP3
• Gene: Pirt Location: Chr11:66802807-66820702 bp, + strand Genetic Position: Chr11, 40.59 cM
• Alliance: Pirt^tm2Xzd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:213013
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The GCaMP3 reporter and neomycin resistance cassette replaced the entire coding sequence. The GCaMP consists of, from 5' to 3', a poly-histidine tag (6XHis), 13-residue peptide of myosin light chain kinase (M13), a circularly permutated eGFP interrupted at residue 145, calmodulin (CaM), and a polyA recognition sequence. Mutations resulting in increased brightness (D180Y and V93I), thermal stability (V163A, and S175G) and GFP dimerization prevention (A206K) were introduced. To further stabilize GCaMP and increase fluorescence, four more mutations (R2V, M66K, T116V and N363D) were introduced, resulting in the creation of GCaMP3. (J:213013)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pirt Mutation: 13 strains or lines available

References

• Original: J:213013 Kim YS, et al., Central terminal sensitization of TRPV1 by descending serotonergic facilitation modulates chronic pain. Neuron. 2014 Feb 19;81(4):873-87
• All: 26 reference(s)