Dact1tm2b(EUCOMM)Hmgu
Targeted Allele Detail
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Symbol: |
Dact1tm2b(EUCOMM)Hmgu |
Name: |
dishevelled-binding antagonist of beta-catenin 1; targeted mutation 2b, Helmholtz Zentrum Muenchen GmbH |
MGI ID: |
MGI:5620879 |
Synonyms: |
Dact1LacZ |
Gene: |
Dact1 Location: Chr12:71356658-71366881 bp, + strand Genetic Position: Chr12, 29.39 cM
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Alliance: |
Dact1tm2b(EUCOMM)Hmgu page
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IMPC: |
Dact1 gene page |
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Mutant Cell Line: |
HEPD0545_2_A04 |
Germline Transmission: |
Earliest citation of germline transmission:
J:211189
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Parent Cell Line: |
JM8.N4 (ES Cell)
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Strain of Origin: |
C57BL/6N
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Project Collection: |
EUCOMM
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Allele Type: |
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Targeted (Null/knockout, Reporter) |
Mutations: |
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Insertion, Intragenic deletion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 71359010 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 71359851. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a reporter knockout mouse.
(J:157065, J:211189)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Dact1 Mutation: |
21 strains or lines available
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J:211189 used HEPD0545_2_A04 mutant ES cells to create this allele.
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Original: |
J:211189 Suzuki D, et al., Expression analysis of Dact1 in mice using a LacZ reporter. Gene Expr Patterns. 2014 May;15(1):21-30 |
All: |
2 reference(s) |
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