Dact1^{tm2b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Dact1^{tm2b(EUCOMM)Hmgu}
• Name: dishevelled-binding antagonist of beta-catenin 1; targeted mutation 2b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5620879
• Synonyms: Dact1^LacZ
• Gene: Dact1 Location: Chr12:71356658-71366881 bp, + strand Genetic Position: Chr12, 29.39 cM
• Alliance: Dact1^{tm2b(EUCOMM)Hmgu} page
• IMPC: Dact1 gene page

Mutation origin

• Mutant Cell Line: HEPD0545_2_A04
• Germline Transmission: Earliest citation of germline transmission: J:211189
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 71359010 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 71359851. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a reporter knockout mouse. (J:157065, J:211189)

Expression

In Mice Carrying this Mutation:

128 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dact1 Mutation: 18 strains or lines available

Notes

J:211189 used HEPD0545_2_A04 mutant ES cells to create this allele.

References

• Original: J:211189 Suzuki D, et al., Expression analysis of Dact1 in mice using a LacZ reporter. Gene Expr Patterns. 2014 May;15(1):21-30
• All: 2 reference(s)