Ldha^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ldha^{tm1c(EUCOMM)Wtsi}
• Name: lactate dehydrogenase A; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5619796
• Synonyms: LDH^fl
• Gene: Ldha Location: Chr7:46491698-46505051 bp, + strand Genetic Position: Chr7, 30.6 cM
• Alliance: Ldha^{tm1c(EUCOMM)Wtsi} page
• IMPC: Ldha gene page

Mutation origin

• Mutant Cell Line: EPD0070_2_G04
• Germline Transmission: Earliest citation of germline transmission: J:214948
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 46497932 of Chromosome 7 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon 3 at position 46498818. The critical exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. (J:214948)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ldha Mutation: 34 strains or lines available

References

• Original: J:214948 Wang YH, et al., Cell-state-specific metabolic dependency in hematopoiesis and leukemogenesis. Cell. 2014 Sep 11;158(6):1309-23
• All: 37 reference(s)