Lcp2^tm2.1Mal
Targeted Allele Detail

Summary

• Symbol: Lcp2^tm2.1Mal
• Name: lymphocyte cytosolic protein 2; targeted mutation 2.1, Bernard Malissen
• MGI ID: MGI:5617470
• Synonyms: Lcp2^tm2Mal, Slp76^OST, SLP-76^OST
• Gene: Lcp2 Location: Chr11:33996928-34042281 bp, + strand Genetic Position: Chr11, 19.43 cM
• Alliance: Lcp2^tm2.1Mal page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:209882
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Epitope tag, No functional change)
• Mutation: Insertion
• Mutation details: Sequence for a Gly-Ser-Gly spacer, a OneSTrEPtag (OST), two STOP codons, a loxP site, the mouse Ace (angiotensin I converting enzyme (peptidyl-dipeptidase A) 1) promoter, the cre recombinase gene, a neomycin resistance gene cassette, and a second loxP site, was inserted in-frame at the 3 end of the gene coding sequence in exon 21. Cre-mediated self-excision removed the cre+neo cassette in the male germline. The fusion of the OST to the protein can be used in affinity purification. The presence of this tag does not affect protein expression. (J:209882)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lcp2 Mutation: 41 strains or lines available

References

• Original: J:209882 Roncagalli R, et al., Quantitative proteomics analysis of signalosome dynamics in primary T cells identifies the surface receptor CD6 as a Lat adaptor-independent TCR signaling hub. Nat Immunol. 2014 Apr;15(4):384-392
• All: 2 reference(s)