Gt(ROSA)26Sor^{tm1(CAG-ECFP/EYFP*)Feil}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-ECFP/EYFP*)Feil}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Robert Feil
• MGI ID: MGI:5614756
• Synonyms: Gt(ROSA26)Sor^{tm1(CAG-cGi500)Feil}, R26-CAG-cGi500(L2)
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-ECFP/EYFP*)Feil} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:213388
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct inserted into the locus a CAG promoter, floxed membrane-targeted tandem-dimer Tomato (mT) reporter gene, an improved sensor cGMP indicator (cGi500; containing an FRET-activated, cGMP-sensitive ECFP/EYFP fusion) and an FRT-flanked neomycin resistance cassette. Cre-mediated recombination can remove the Tomato reporter gene to allow the expression of the cGMP indicator in a time- and cell type-specific manner. The cGMP indicator has an EC50 of 500 nmol/L. (J:213388)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 944 strains or lines available

References

• Original: J:213388 Thunemann M, et al., Transgenic mice for cGMP imaging. Circ Res. 2013 Aug 2;113(4):365-71
• All: 2 reference(s)