Snap25^tm3.1Hze
Targeted Allele Detail

Summary

• Symbol: Snap25^tm3.1Hze
• Name: synaptosomal-associated protein 25; targeted mutation 3.1, Hongkui Zeng
• MGI ID: MGI:5613588
• Synonyms: Snap25-2A-GCaMP6s, Snap25-2A-GCaMP6s-Delta, Snap25-2A-GCaMP6s-DeltaHygro, Snap25^{tm3.1(GCaMP6s)Hze}
• Gene: Snap25 Location: Chr2:136555373-136624348 bp, + strand Genetic Position: Chr2, 67.56 cM
• Alliance: Snap25^tm3.1Hze page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct contained (from 5' to 3') a partial sequence spanning intron 6 through intron 7, an frt3 site within intron 7, a partial exon 8 sequence up to but not including the endogenous translational stop codon, a T2A peptide cleavage signal in-frame with the coding sequence, a GCaMP6s gene (slow variant calcium indicator; an ultrasensitive detector of single neuronal action potentials with slow response kinetics) that is in-frame with the coding sequence, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-hygromycin resistance gene-SV40polyA cassette (with an mRNA splice donor-frt5 site-mRNA splice acceptor in the hygromycin gene), and an AttP site. PhiC31-mediated recombination removed the selection cassette. (J:101977, J:219930)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Snap25 Mutation: 26 strains or lines available

References

• Original: J:219930 Madisen L, et al., Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron. 2015 Mar 4;85(5):942-58
• All: 18 reference(s)