Pdcd2^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Pdcd2^{tm1c(EUCOMM)Wtsi}
• Name: programmed cell death 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5609169
• Synonyms: Pdcd2^flox
• Gene: Pdcd2 Location: Chr17:15739472-15747560 bp, - strand Genetic Position: Chr17, 8.95 cM
• Alliance: Pdcd2^{tm1c(EUCOMM)Wtsi} page
• IMPC: Pdcd2 gene page

Mutation origin

• Mutant Cell Line: EPD0372_1_A09
• Germline Transmission: Earliest citation of germline transmission: J:213699
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The cassette is composed of from 5' to 3' an frt site, an En2 splice acceptor cassette containing a beta--galactosidase sequence, and loxP-flanked neomycin cassette, containing a frt site, inserted downstream of exon 1. A third loxP site was inserted downstream of exon 2. The critical exon is thus flanked by loxP sites. FLP mediated recombination removed the FRT-flanked lacZ and neo cassette leaving exon 2 flanked by loxP sites. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:155845, J:213699)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pdcd2 Mutation: 15 strains or lines available

References

• Original: J:213699 Granier CJ, et al., Conditional inactivation of PDCD2 induces p53 activation and cell cycle arrest. Biol Open. 2014;3(9):821-31
• All: 2 reference(s)