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Targeted Allele Detail
Symbol: Cnr1tm1.1Kpm
Name: cannabinoid receptor 1 (brain); targeted mutation 1.1, Ken Mackie
MGI ID: MGI:5607776
Synonyms: S426A/S430A
Gene: Cnr1  Location: Chr4:33924593-33948831 bp, + strand  Genetic Position: Chr4, 16.28 cM
Germline Transmission:  Earliest citation of germline transmission: J:208116
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Allele Type:    Targeted (Not Specified)
Mutation:    Nucleotide substitutions
Mutation detailsAn HA epitope sequence, two mutations that change serines 426 and 430 to alanines (S426A, S430A), and a neomycin phosphotransferase (NeoR) gene flanked by frt sequences were inserted via homologous recombination. Serines 426 and 430 are putative GRK phosphorylation sites that when mutated to alanines are nonphosphorylatable and result in a desensitization resistant form of Cnr1. The frt-flanked NeoR gene was removed via flp-mediated recombination. Southern blot confirmed expression of a 4.2 kb mutant fragment and PCR confirmed expression of the mutant mRNA. Western blot analysis confirmed that protein levels in the brain remain unchanged except for a 15% decrease in the cerebellum. (J:208116)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Cnr1 Mutation:  24 strains or lines available
Original:  J:208116 Morgan DJ, et al., Mutation of Putative GRK Phosphorylation Sites in the Cannabinoid Receptor 1 (CB1R) Confers Resistance to Cannabinoid Tolerance and Hypersensitivity to Cannabinoids in Mice. J Neurosci. 2014 Apr 9;34(15):5152-63
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.16
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