Cacna1h^{tm1.1(epH)Ebou}
Targeted Allele Detail

Summary

• Symbol: Cacna1h^{tm1.1(epH)Ebou}
• Name: calcium channel, voltage-dependent, T type, alpha 1H subunit; targeted mutation 1.1, Emmanuel Bourinet
• MGI ID: MGI:5607172
• Synonyms: Cav3.2-ecliptic-GFP, Cav3.2GFPe KI, Cav3.2-GFP KI
• Gene: Cacna1h Location: Chr17:25593259-25652759 bp, - strand Genetic Position: Chr17, 12.53 cM
• Alliance: Cacna1h^{tm1.1(epH)Ebou} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:220891
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The Cacna1h (also known as Cav3.2) gene was modified by the insertion of the DNA sequence encoding "ecliptic pHlourin" (also called "ecliptic GFP"; a pH-sensitive derivative of wild-type green fluorescent protein), in-frame, into exon 6 and of loxP sites upstream of exon 6 and downstream of exon 7; a frt-flanked neomycin selection cassette upstream of the first loxP site was excised by flp recombinase. In the resultant fusion protein, ecliptic GFP is part of a large extracellular loop preceding the P loop of the first transmembrane domain. Northern blot and in-situ hybridization analyses with Cacna1h and GFP probes and western blot and fluorescence immunohistology analyses with antibodies to GFP and CACNA1H verified expression of the fusion mRNA and protein, respectively, in whole brain, whole dorsal root ganglion (DRG) and DRG sections from homozygous mutant adult mice. Functionality of the fusion protein-derived channels is demonstrated by electrophysiological analysis of live DRG tissue from homozygous mutant mice. Crossing mice with this allele to cre recombinase-expressing mice results in excision of exons 6 and 7 (and the GFP insertion) and production of a non-functional calcium channel. (J:94746, J:220891)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cacna1h Mutation: 98 strains or lines available

References

• Original: J:220891 Francois A, et al., The Low-Threshold Calcium Channel Cav3.2 Determines Low-Threshold Mechanoreceptor Function. Cell Rep. 2015 Jan 14
• All: 5 reference(s)