Pten^tm1.1Whs
Targeted Allele Detail

Summary

• Symbol: Pten^tm1.1Whs
• Name: phosphatase and tensin homolog; targeted mutation 1.1, Wen H Shen
• MGI ID: MGI:5605962
• Synonyms: PTEN^deltaC, Pten^tm1.1Yxy
• Gene: Pten Location: Chr19:32734977-32803560 bp, + strand Genetic Position: Chr19, 28.14 cM
• Alliance: Pten^tm1.1Whs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:211716
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Single point mutation
• Mutation details: This mutation was created by generating targeting constructs containing a 5' homology region with Pten exons 4-5, a neo cassette flanked by two loxP sites (LoxP- Neo-LoxP), and a 3' homology region containing exon 6 with the point mutation (c.565A > T) preceded by a FLAG tag. After homologous recombination in ES cells and injection into C57BL/6 blastocysts, the resulting chimeras were bred with EIIA-Cre transgenic female mice to delete the loxP-flanked neomycin resistance gene (neo) from the targeted allele. The C-terminal truncated Pten protein, is expressed in various tissues including kidney, lung, uterus and heart. (J:211716)

Tumor Data

List all tumor models in MMHCdb carrying Pten^tm1.1Whs

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pten Mutation: 81 strains or lines available

Notes

The mutation was generated in an unspecified ES cell line derived from C57BL/6 and 129S6/SvEvTac. J:211716)

References

• Original: J:211716 Sun Z, et al., PTEN C-terminal deletion causes genomic instability and tumor development. Cell Rep. 2014 Mar 13;6(5):844-54
• All: 1 reference(s)