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Dlg4tm1.1Hnz
Targeted Allele Detail
Nomenclature
Symbol: Dlg4tm1.1Hnz
Name: discs large MAGUK scaffold protein 4; targeted mutation 1, Haining Zhong
MGI ID: MGI:5588003
Synonyms: PSD-95-ENABLED
Gene: Dlg4  Location: Chr11:69908029-69938107 bp, + strand  Genetic Position: Chr11, 42.96 cM, cytoband B4
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:218842
Parent Cell Line:  Other (see notes) (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6J)F1
Mutation
description
Allele Type:    Targeted (Conditional ready, Reporter)
Mutation:    Insertion
 
Mutation detailsThe last two exons of the gene (exons 19 and 20), including the 3' UTR, were duplicated and inserted downstream of the polyadenylation signal and a neomycin selection cassette flanked by FRT sites. Two loxP sites were inserted to flank the native exons 19 and 20 as well as a neomycin stop cassette. The sequence for mVenus was fused in frame to the end of the coding sequence in exon 20. The FRT-flanked neomycin cassette was excised through crosses with a germline flp strain. (J:218842)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Dlg4 Mutation:  28 strains or lines available
Notes
ES cell line = G1 (129S6/SvEvTac x C57BL/6J)F1.
References
Original:  J:218842 Fortin DA, et al., Live imaging of endogenous PSD-95 using ENABLED: a conditional strategy to fluorescently label endogenous proteins. J Neurosci. 2014 Dec 10;34(50):16698-712
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/30/2021
MGI 6.17
The Jackson Laboratory