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Transgene Detail
Symbol: Tg(Csf1r*-GAL4/VP16,UAS-ECFP)1Hume
Name: transgene insertion 1, David A Hume
MGI ID: MGI:5587942
Synonyms: Csf1r-Gal4VP16/UAS-ECFP, MacBlue
Transgene: Tg(Csf1r*-GAL4/VP16,UAS-ECFP)1Hume  Location: unknown  
Strain of Origin:  (C57BL/6J x CBA)F2
Transgene Type:    Transgenic (Reporter)
Mutation:    Insertion
Mutation detailsTwo transgenes inserted at a single locus. One transgenic construct is designed with a colony stimulating factor 1 receptor (Csf1r) promoter, lacking the 158 bp (-454 to -298 relative to ATG) trophoblast transcription start sites and a TATA-box. This mutated Csf1r drives expression of a "Gal4 driver" (containing a yeast GAL4 transcriptional activator fused to herpes simplex virus protein (VP16)). A "Gal4-responder" plasmid is designed to contain an enhanced cyan fluorescent protein (ECFP) uder the control of a yeast upstream activating sequence (UAS) Gal4-dependent promoter. The founder line with approximately 5% ECFP+ leukocytes was selected for characterization. (J:131223)
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Original:  J:131223 Ovchinnikov DA, et al., Expression of Gal4-dependent transgenes in cells of the mononuclear phagocyte system labeled with enhanced cyan fluorescent protein using Csf1r-Gal4VP16/UAS-ECFP double-transgenic mice. J Leukoc Biol. 2008 Feb;83(2):430-3
All:  11 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.16
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