Ffar4^{tm2e(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Ffar4^{tm2e(EUCOMM)Hmgu}
• Name: free fatty acid receptor 4; targeted mutation 2e, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5581410
• Gene: Ffar4 Location: Chr19:38085527-38102711 bp, + strand Genetic Position: Chr19, 32.73 cM
• IMPC: Ffar4 gene page

Mutation origin

• Mutant Cell Lines: HEPD0977_2_A06, HEPD0977_2_A07, HEPD0977_2_B03, HEPD0977_2_B04, HEPD0977_2_B05, HEPD0977_2_B06, HEPD0977_2_B07, HEPD0977_2_B08, HEPD0977_2_C05, HEPD0977_2_C06, HEPD0977_2_D07, HEPD0977_2_D12, HEPD0977_2_F07, HEPD0977_2_H01, HEPD0977_2_H02
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion Vector: Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo
• Mutation details: The Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo cassette was inserted at position 38085703 of Chromosome 19 (Build GRCm39) within an endogenous exon, creating an artificial intron that splits the original exon into two artificial exons, where the downstream artificial exon is critical. The cassette is composed of the intron of the Ifitm2 gene containing an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other EUCOMMTOOLs alleles can be found at http://www.knockoutmouse.org/about/eucommtools/vectors. (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ffar4 Mutation: 22 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 1 reference(s)