Tg(Myh6-ADCY8)7Hj
Transgene Detail

Summary

• Symbol: Tg(Myh6-ADCY8)7Hj
• Name: transgene insertion 7, Jacques Hanoune
• MGI ID: MGI:5578165
• Synonyms: AC8TG, AC8 transgene, alpha-MHC-AC8-SV40 intron/pA transgene
• Transgene: Tg(Myh6-ADCY8)7Hj Location: unknown
• Alliance: Tg(Myh6-ADCY8)7Hj page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA)F2

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Myh6-ADCY8)7Hj expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ADCY8 (114)

  Adcy8

  (MGI:1341110)

• Tg(Myh6-ADCY8)7Hj expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Myh6 (MGI:97255)

• Mutation details: The transgene contains the promoter of the mouse myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6) gene joined to a cDNA encompassing the entire coding sequence of human adenylate cyclase 8 - which normally is expressed in the nervous system and is activated by Ca⁺ - followed by the SV40 intron and polyadenylation signal. Two transgenic founder lines were identified; both lines were estimated by dot-blot analysis to carry 7 to 8 copies of the transgene. Northern blot analysis of total RNA extracted from brain, heart, kidney and skeletal muscle of transgenic mice confirmed expression only in heart. Adenylate cyclase activity is increased at least 7-fold in cardiac membranes of transgenic vs. nontransgenic control mice. (J:62373)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:62373 Lipskaia L, et al., Enhanced cardiac function in transgenic mice expressing a Ca(2+)-stimulated adenylyl cyclase. Circ Res. 2000 Apr 14;86(7):795-801
• All: 4 reference(s)