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Transgene Detail
Symbol: Tg(Ube3a)1Mpan
Name: transgene insertion 1, Matthew Anderson
MGI ID: MGI:5576240
Synonyms: FLAG-Ube3a
Transgene: Tg(Ube3a)1Mpan  Location: unknown  
Strain of Origin:  FVB/NJ
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
Tg(Ube3a)1Mpan expresses 1 gene
Mutation detailsThe bacterial artificial chromosome (BAC) clone RP24-178G7 contains the entire ubiquitin protein ligase E3A locus (Ube3a) as well as ~63 kbp of 5' flanking sequence and ~21 kbp of 3' flanking sequence. DNA sequences encoding three FLAG epitope tags and two translational stop codons were inserted in-frame at the 3' coding/untranslated boundary of exon 12 of the gene on the BAC via homologous recombination/BAC recombineering. A single frt site remains just downstream of the 3xFLAG region. Two founder lines with independent integration sites were established; lines 1 and 2. Both founder lines exhibit the same expression pattern in the tissues examined (although line 2 may have slightly lower levels). Animals from line 1 were more extensively characterized. Ube3a expression was confirmed by Western blot of cortical lysates. (J:178319)
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Original:  J:178319 Smith SE, et al., Increased gene dosage of Ube3a results in autism traits and decreased glutamate synaptic transmission in mice. Sci Transl Med. 2011 Oct 5;3(103):103ra97
All:  7 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.17
The Jackson Laboratory