Lmna^tm1.2Yxz
Targeted Allele Detail

Summary

• Symbol: Lmna^tm1.2Yxz
• Name: lamin A; targeted mutation 1.2, Yixian Zheng
• MGI ID: MGI:5575530
• Synonyms: Lmna^delta
• Gene: Lmna Location: Chr3:88388455-88413842 bp, - strand Genetic Position: Chr3, 38.84 cM
• Alliance: Lmna^tm1.2Yxz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:211369
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 2. An FRT-flanked puromycin resistance cassette with a 3' loxP site was inserted downstream of exon 2. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination removed exon 2. Immunofluorescence confirmed the absence of lamin A and C in mouse embryonic fibroblasts. (J:211369)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lmna Mutation: 82 strains or lines available

References

• Original: J:211369 Kim Y, et al., Generation and characterization of a conditional deletion allele for Lmna in mice. Biochem Biophys Res Commun. 2013 Oct 11;440(1):8-13
• All: 1 reference(s)