Tg(Hspa1b-mPlum)AAmpla
Transgene Detail

Summary

• Symbol: Tg(Hspa1b-mPlum)AAmpla
• Name: transgene insertion 1, Anna Planas
• MGI ID: MGI:5575510
• Synonyms: Hsp70-mPlum
• Transgene: Tg(Hspa1b-mPlum)AAmpla Location: unknown
• Alliance: Tg(Hspa1b-mPlum)AAmpla page

Transgene origin

• Strain of Origin: (C57BL/6J x CBA/J)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: The transgene contains a 516-bp promoter fragment of the stress-inducible mouse Hspa1b gene (also called Hsp70), including 284 bp of 5' flanking sequence and the 5' untranslated region, separated by a Kozak consensus sequence (ACCACC) and a rabbit beta-globin intron from the mPlum open reading frame, which is followed by the SV40 early polyadenylation signal. Brains of transgenic mice in which focal ischemia had been induced one day prior exhibited a fluorescence resonance imaging (FRI) signal coincident with expression of endogenous Hspa1b as determined by immunohistochemistry, and mPlum fluorescence was observed by in vivo confocal microscopy in penumbra neurons and in blood vessels at the ischemic core. mPlum expression does not affect neuronal or penumbral viability. (J:211474)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Three female transgenic founder mice transmitted the transgene to their offspring.

References

• Original: J:211474 de la Rosa X, et al., In vivo imaging of induction of heat-shock protein-70 gene expression with fluorescence reflectance imaging and intravital confocal microscopy following brain ischaemia in reporter mice. Eur J Nucl Med Mol Imaging. 2013 Feb;40(3):426-38
• All: 1 reference(s)