About   Help   FAQ
Tg(Hspa1b-mPlum)AAmpla
Transgene Detail
Nomenclature
Symbol: Tg(Hspa1b-mPlum)AAmpla
Name: transgene insertion 1, Anna Planas
MGI ID: MGI:5575510
Synonyms: Hsp70-mPlum
Transgene: Tg(Hspa1b-mPlum)AAmpla  Location: unknown  
Transgene
origin
Strain of Origin:  (C57BL/6J x CBA/J)F2
Transgene
description
Transgene Type:    Transgenic (Inserted expressed sequence, Reporter)
Mutation:    Insertion
 
Mutation detailsThe transgene contains a 516-bp promoter fragment of the stress-inducible mouse Hspa1b gene (also called Hsp70), including 284 bp of 5' flanking sequence and the 5' untranslated region, separated by a Kozak consensus sequence (ACCACC) and a rabbit beta-globin intron from the mPlum open reading frame, which is followed by the SV40 early polyadenylation signal. Brains of transgenic mice in which focal ischemia had been induced one day prior exhibited a fluorescence resonance imaging (FRI) signal coincident with expression of endogenous Hspa1b as determined by immunohistochemistry, and mPlum fluorescence was observed by in vivo confocal microscopy in penumbra neurons and in blood vessels at the ischemic core. mPlum expression does not affect neuronal or penumbral viability. (J:211474)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Notes
Three female transgenic founder mice transmitted the transgene to their offspring.
References
Original:  J:211474 de la Rosa X, et al., In vivo imaging of induction of heat-shock protein-70 gene expression with fluorescence reflectance imaging and intravital confocal microscopy following brain ischaemia in reporter mice. Eur J Nucl Med Mol Imaging. 2013 Feb;40(3):426-38
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
07/29/2020
MGI 6.15
The Jackson Laboratory