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Tg(CMV-EYFP/DsRed2)1Mklv
Transgene Detail
Nomenclature
Symbol: Tg(CMV-EYFP/DsRed2)1Mklv
Name: transgene insertion 1, Andrey Mikhailov
MGI ID: MGI:5573173
Synonyms: EYFP-CCS-DsRed, EYFP-DEVD-DsRed FRET, FRET-casp3, TandoMice, Yellow Fluorescent Protein(EYFP)-caspase-3 cleavage site(CCS)-Red Fluorescent Protein (DsRed)
Transgene: Tg(CMV-EYFP/DsRed2)1Mklv  Location: unknown  
Transgene
origin
Strain of Origin:  Not Specified
Transgene
description
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
 
Mutation detailsThe cytomegalovirus enhancer/promoter drives expression of a chimeric protein in which enhanced yellow fluorescent protein (EYFP) is separated from Discosoma red fluorescent protein 2 (DsRed2) by a 19-amino acid peptide linker that includes the capase-3 cleavage site (Asp-Glu-Val-Asp). When cells expressing the intact chimeric protein are excited with a 450-490 nm light or with a 488 nm argon laser, they strongly fluoresce red due to fluorescence resonance energy transfer (FRET) from donor EYFP to acceptor DsRed2. Upon caspase 3 activation, cleavage of the joining peptide chain results in increased EYFP fluorescence and concomitant decreased DsRed fluorescence, which is correlated with increased pro-caspase 3 processing. This serves as a sensitive reporter of caspase 3 activation upon induction of apoptosis. (J:82809, J:209202)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
12/03/2019
MGI 6.14
The Jackson Laboratory